SpectraMax Multi-Mode Microplate Readers. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Optical density was measured at 570 nm using a microplate reader (SpectraMAX 340, Molecular Devices Co. The mixture was centrifuged for 10 min at 10,000 rpm, and BSA concentration in the supernatant was determined using bicinchoninic acid (BCA) assay. The plate was covered and incubated for one hour at room temperature. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. With SOFTmax PRO, the contents of the wells in a microplate can be mixed the industry-leading microplate reader software for the SpectraMax Plus 384 system, allows complete customization of data collection and analysis. The absorbance was read at the wavelength of 570 nm using a microplate spectrophotometer (SpectraMax 340, Molecular Devices, CA, USA). Absorbance was measured at 450 nm by using a SpectraMax 340 microplate spectrophotometer (Molecular Device). Narrow bandwidths deliver increased measurement accuracy and linearity over a wide range of. Softmax PRO v5. DNA extraction from blood meal ELISA homogenate. 6. #N207) LAL Reagent Water (Lonza cat. The light features a slim profile to prevent. ELISAs were read with a Spectramax 340 from Molecular Devices (Sunnyvale, CA, USA). Absorbance was measured at 450 nm with ELISA Reader (SpectraMax 340, Molecular Device Co. SpectraMax Multi-Mode Microplate Readers. The VersaMax and SpectraMax 190 read 96-well microplates. All ELISA plates were analyzed using a SpectraMax 340 PC microplate reader (Molecular Devices). 303 unable to cal dark current. 2 - 3. Lactate Dehydrogenase (LDH) Release. SpectraMax Plus 384 measuring instruments pdf manual download. Oxidative stress induction by H 2 O. 340/70 nm. The growth. SpectraMax 340PC384 reads 96-well plates and 384-well plates and strip-well plates. edu Contact Phone: 216-368-8962. Each SpectraMax® 340 PC 384 Microplate Reader has an. The inhibition of ALDH activity by compounds and the IC50 curves were determined by measuring the formation of NAD(P)H spectrophotometrically at 340 nm (molar extinction coefficient of 6200 M −1 cm −1) on a Beckman DU-640 as well as a Spectramax 340 PC spectrophotometer (GMI, Ramsey, NJ, USA) using a purified. . Molecular Devices Corporation 1311. SpectraMAX 340 microtiter plate reader. The screening was initiated by adding 10 μL p-nitrophenyl phosphate (pNPP), and the catalysis of pNPP (1 mM) was continuously monitored on SpectraMax 340 microplate reader (Molecular Devices, Sunnyvale, CA, USA) at 405 nm for 20 min at 37 °C. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Call 1-800-745-2710. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. 4. Manufacturer: Molecular Devices Model: SpectraMax The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurement in the visible range. 1% N-1-naphtylethylenediamine dihydrochloride, 2. The centrifuged solution was transferred to a different microtitre plate and the optical density (OD) of the change in color was measured using a plate reader (SpectraMAX 340 Tunable Microplate Reader; Molecular Devices Ltd. Endpoint, kinetic, spectrum. Unit has several areas of damaged plastic. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. Following HRP-conjugated secondary antibodies, goat anti-rat IgG (Kirkegaard & Perry. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. The SpectraMax 340PC384 Microplate reader supports various absorbance assay categories, such as protein quantitation, endotoxin detection, microbial growth, ELISAs. The loading equaled the mass of BSA per mass of particles. After washing, the dye was eluted with 3% HCl, and the absorbance measured with a SpectraMax 340 microtiter plates reader at 665 nm. After 72 h, 20 μl of 5 mg/ml MTT solution was added to each well and plates were incubated for 3 h at 37°C. Analysis of ALA by Ehrlich’s Reagent Assay. Molecular Devices SpectraMax Gemini XS Microplate Reader. The absorbance at 405 nm was determined using a microplate reader (spectraMAX 340). The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. 340-850 nm: Wavelength Selection: Monochromator, turnable in 1 nm increments:. The concentration of NO in the cells was determined by measuring the amount of nitrite, a relatively stable oxidation product of. Not all manufacturers' microplates are the same with regard to design, materials, orAbsorbance at 405 nm (Abs 405) was obtained for both plates before (background) and after 1-h incubation at 37°C using a microplate reader (SpectraMax 340 PC384, Molecular Devices). The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. The absorbance at 540 nm was determined in a spectrophotometer (SPECTRAmax 340, Molecular Devices). ): 8. The area under the curve (AUC) for the comparison of the growth rates was determined using. View the Molecular Devices website. Immunofluorescence and Aggregation Analysis. The optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. Molecular Devices Corporation 1311. Data were expressed as the mean percent viable cell vs. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. Vessel Sprout Formation Assay. Data are expressed as percent of maximum binding for each protein. The SpectraMax® QuickDrop™ Micro-Volume Spectrophotometer . Lactate dehydrogenase (LDH) release assay Cytotoxicity was determined by measuring the release of LDH. Permeability of the monolayer was verified by adding 1% Evans Blue dye (Thermo Fisher Scientific, Inc. Amino acid analysis was performed on a Pico-Tag amino acid analyzer (Wat er System) as. DNA/RNA concentration is commonly estimated by dividing the A260 value by the 1-cm absorptivity value (or multiplying by its reciprocal). SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. This Molecular Devices SPECTRAmax 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. The values of IC 50 represent concentrations of compounds toA microplate reader (SpectraMax M5; Molecular Devices, Sunnyvale, CA) was used to measure the absorbance at 340 nm for the collected BPW (6) Stabilization protects islet integrity during respirometry in the Oroboros Oxygraph-2K analyzer Islets December 31, 2022 Justin J. The assay was carried out in a 96-well plate in a final volume of 100 µl containing 50 mmol/l Tris-HCl, pH 7. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. The increase in fluorescence was determined for 20 min by excitation at 520 nm and emission at 545 nm in a. Create custom fluorescence assays without having to purchase expensive filter sets. Absorbance was read at 405 nm on a microplate reader (SPECTRAmax 340 PC). 2. Absorbance measurements were recorded using a SpectraMax 340 (Molecular Devices) at 570nm on the indicated days (Cell Proliferation Kit I; Roche). No power cord or accessories included. Whether you are looking for an intuitive, easy-to-use reader for your busy, multi-user lab, or an upgradeable system that can adapt to support your lab’s new projects andSPECTRAmax ® PLUS 384 Microplate Spectrophotometer. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax. 00 USD. 2 g/L solution; dilute 1:10 in HBSS. The values were compared against a standard curve that was generated using known concentrations of IL6 to calculate concentration in the. 7. 25–0. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. TDP-43 stress granular cells were treated with 70% ice-cold. LDH release was measured with a commercial kit following the manufacturer’s instructions (LDH Cytotoxicity Detection Kit, TaKaRa). TGFα release was calculated as described in the Results. After adding the stop solution, the absorbance at 450 nm with a wavelength correction at 540 nm was measured with a SpectraMax 340 plate reader (Molecular Devices, San. As a common indicator of equine T cell activation, cytokine induction. Cross sections of mouse skin was prepared and stained as previously described . With SOFTmax PRO, the contents of the wells in a microplate can be mixed SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. TDP-43 stress granular cells were treated with 70% ice-cold. Alves, Amelia K. 30. The absorbance was measured at 405 nm on a SpectraMax 340 (Molecular Devices). IC 50 values were determined by mathematical curve-fitting (SoftMaxPro Molecular Devices) and were defined as the drug concentration leading to 50% inhibition of net cell mass. When necessary, samples were. Cell viability was determined using the blue formazan assay in which colorless 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) is metabolized to a blue product by mitochondrial dehydrogenases. 340 nm. Water – distilled water or equivalent. Absorbance plate reader, temperature control, robotics-compatible, 384 well plates, 340-850 nm. View the LabX ads below to find Molecular Devices SpectraMAX plate readers for sale and. SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Molecular Devices Spectramax 340PC instructions. Related Products: SpectraMax 340 Absorbance Microplate Reader, Molecular Devices SpectraMax 384 Plus UV/Vis Microplate Reader, Molecular DevicesGETTING THE MOST FROM YOUR MOLECULAR DEVICES MICROPLATE SYSTEMS applications guide to microplate systems applications guide to microplate systems450 nm using a SpectraMax 340 microplate reader (Molecular Devices, Sunnyvale, CA, USA). 4- to 5-weeks old female BALB/c mice were purchased from Charles River Laboratories (QC, Canada). Accurate quantitation The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurement in the visible range. The optical density of each well was determined immediately using a microplate reader (SpectraMax-340, Molecular Devices) set to 450 nm. using SpectraMax i3 (Molecular devices, San Jose, USA). With SOFTmax PRO, the contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, making it possible to. Time-resolved fluorescence was measured on the SpectraMax i3x and SpectraMax iD5 readers using preconfigured protocols in SoftMax® Pro Software (see Tables 2 and 3 for instrument-specific settings). Absorbance measurements were recorded using a SpectraMax 340 (Molecular Devices) at 490 nm on the indicated days (Cell Proliferation Kit I; Roche). LAL Reagent Grade ™ Multi-well Plates (Lonza cat. Microplate Readers. Includes: Computer Loaded with Softmax Pro 5. , Oxon, UK). Assay setup for a 384-well low-volume plate. Pharmacokinetic and pharmacodynamic analysis. SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. The drawer will open and it may close, if is doesn't, close it using the DRAWER button found on the Spectramax control panel (pictured in purple on. The plate was covered and incubated for one hour at room temperature. 5 mL of 1 M acetate buffer (pH 4. SpectraMax ® Plus 384 optics. Protein electrophoresis system (BioRad, Hercules, CA) and Odyssey Infrared Imaging System (Li-Cor Biosciences, Lincoln, NE) were used in western blot. Values are expressed as the mean s ± SD of 3 separate experiments, each performed in triplicate. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). View the Molecular Devices website. , Sunnyvale, CA). Other technologies such as imaging. Table 1. 0 3. Cell viability wasDescription Microplates The SpectraMax Multi-Mode Microplate Reader can accommodate SBS- standard 6-well to 384-well microplates and strip wells. 0 2. The SPECTRAmax 340PC can measure. 5. The results of ELISA optical density (OD) values in duplicates were averaged for the following analyses. This is a used Molecular Devices SpectraMax 340PC Microplate Spectrophotometer. Effect of hypoxia on natriuretic peptides and VEGF production in AC16 cells. Finally, after another 45 min of decolorization, 100 µL of the solution was transferred to a new well and the amount of crystal-violet stain in the decolorizing solution was measured with a microtiter plate reader (SpectraMAX 340 Adjustable Microplate Reader; Molecular Devices Ltd. Or scan up to 384 wells at any time to determine the best assay conditions. The drawer will open and it may close, if is doesn't, close it using the DRAWER button found on the Spectramax control panel (pictured in purple on. The maximum specific growth rate μ was calculated from the exponential growth phase ( 52 ) and plotted against the concentration of the different drugs. With a wide range of on-board features and capabilities, thermoax is ideally suited for use in modern laboratories involved in many applications. To. Molecular Devices’ PathCheck® sensor technology. The reaction mixture consists of 100 mM potassium phosphate buffer (pH 7. The protein concentration was determined spectrophotometrically at 280 nm in a 1 cm quartz cuvette using a SPECTRAmax-340 UV–vis spectrophotometer. 314 low lamp level warning. \r plates can be used in the SPECTRAmax PLUS 384. 340/80 nm excitation with 616/10 nm emission range Optimized for use with ScanLater Western Blot Detection cartridge for the SpectraMax® i3, i3x, and Paradigm® Multi-Mode Readers Optimized for use with the enhanced TRF detection module for the SpectraMax iD5 Multi-Mode Microplate ReaderThe optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. EX range: 340/80 nm EM range: 616/10 nm High fg levels of Streptavidin High fg levels of Streptavidin 2 AlphaScreen. SpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro data analysis software. SPECTRAMAX 340. The SpectraMax i3x reader provides the flexibility to add novel detection capabilities without the need for service engineers or costly downtime—it grows with you as your research areas expand. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. SPECTRAmax 340PC 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. The optical system of the SpectraMax 340PC Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). Call 1-800-745-2710. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening SoftMax® Pro 7. The. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. WST regents of 10% were added to each well, and absorbance was determined at 450 nm using an ELISA reader (SpectraMax® 340, Molecular Device Co. 2. The absorbance values were measured at 450 nm using a SpectraMax® 340 plate reader (Molecular Devices, Sunnyvale, CA, USA). Data were expressed as the mean percent viable cell vs. SpectraMax190,340PC384,Plus384,VersaMaxMicroplateSpectrophotometersUserGuide 4 0112-0126D SafetyInformation. 1% N-1-naphtylethylenediamine dihydrochloride, 2. It includes both a lamp and a new lamp holder. Subsequent replacements require only the lamp (N-34-LB) be replaced. Please refer to photos for further. The relative cell viability was determined as the percentage ratio of the optical densities in the medium (containing the nanoprobes at each concentration) to that of the fresh control medium. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. automated SpectraMAX 340 (Molecular Devices, Sunnyvale, CA, USA) microtiter plate reader. Hydrogen peroxide (H 2 O 2) was used to establish an intracellular oxidative. Tunable for filterless flexibility. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. Donor: 620 nm. Absorbance was read at 405 nm on a microplate reader (SPECTRAmax 340 PC). After 24 h, 48 h, 72 h or 96 h, cell viability was measured by CellTiter 96® AQueous One Solution cell proliferation assay (Promega, Madison, WI, USA). 10× trypsin 0. The cells were pretreated, serum starved for 24 h, and then treated with NK-cdM priora SpectraMAX 340VR microplate reader (Molecular Devices, Sunnyvale, CA, USA). VersaMax reads 96-well and strip-well plates. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. TRF-based with 340/80 nm EX and 616/10 nm EM 0200-7027 EX range: 340/80 nm EM range: 616/10 nm High fg levels of Streptavidin High fg levels of Streptavidin 2 AlphaScreen AlphaScreen and AlphaLisa detection using 1 W 680 nm EX laser diode and a 570 nm (100) EM filter Pick best speed, sensitivity, and price for your needs Table 1. A 340 μL of 50 mM borate buffer (pH 9), 240 μL methanol, 10 μL standard solution or sample, and 20 μL of 0. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. Acceptor: 665nm. The Gemini EM Reader with dual monochromators allows researchers to try new and novel dyes without having toThe absorbance was determined to be 450 nm using an ELISA reader (SpectraMax® 340, Molecular Device Co. 3T3 cells were cultured in DMEM supplemented with 10% FCS and 1% antibiotics (10,000 units/ml penicillin and 10,000 μg/ml streptomycin in 0. Description 1. 1 mg mL −1 CDDP loaded A-CHAM, B-CHAM, C-CHAM or D-CHAM. Molecular Devices SPECTRAmax. The ABS reader ranges from. SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. Statistical analysis. 305 reference level saturation. , Sunnyvale, CA) after incubation of solution mixture at 37 °C for 2 h 30 min in the dark. SpectraMax i3x reader measures absorbance, fluorescence, & luminescence with user-upgradeable application including western blot imaging, AlphaScreen, fast kinetics along with injectors plus additional detection modes. Donor filter: 616/10 nm. P. , Fremont, CA, USA). spectramax i3 multi-mode platform, spectramax i3, spectramax i3 system, scanlater western blot system, spectramax minimax imaging cytometer, softmax pro software, metamorph software, spectramax i3 platform. 5 g/L-EDTA 0. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideMolecular Devices SpectraMax Gemini XS Microplate Fluorometer $2,800. Details. 340 to 740 nm (linear variable dichroic) 220 to 1000 nm for abs (spectrometer) Plate Formats: 6 to 384-well plates, LVis Plate with sixteen 2 µL microspots. 30 µs. SpectraMax® M2/M2e user guide a multi-detection microplate reader with two-mode cuvette port SpectraMax M2e user guide cover 1 1 4/21/06 9:53:28 AM. SpectraMax Multi-Mode Microplate Readers. 3 0. StakMax Microplate Handling Stacker. Products : SpectraMax Plus 384 reader,. 306 plate air cal. Products : SpectraMax Plus 384 reader, SpectraMax 190 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax 250 (Obsolete), SpectraMax 340 (Obsolete), VersaMax reader How does stray light affect my OD reading? The basic problem caused by stray light (light you don't want, getting into the read) is a falsely low OD seen. Microplate Readers. 1 mL of acetylacetone. Money Saved: $340. Molecular Devices SpectraMax M5e Microplate Reader $26,500. PathCheck SensorThe optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. 2 were considered negative for IgM antibody. All strains were tested three times and the results averaged. 30. 1% Chaps, 10 m M dithiothreitol, and 10% sucrose. Wavelength Range. The SpectraMax® iD3 Multi Mode Microplate . 340 nm / 70 nm. Briefly, cells were seeded into a. Molecular Devices ThermoMax is a high-efficiency, temperature-regulated, 96-well microplate reader. IC 50 values were determined by mathematical curve-fitting (SoftMaxPro Molecular Devices) and were defined as the drug concentration leading to 50% inhibition of net cell mass. Changes in cell viability were reflected by changes in optical density detected at 450 nm, which was measured using a spectrophotometer microplate reader (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). The absorbances were being measured at 515 nm. Wavelength(s) 340 to 850 nm; Add to Compare List. The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets users determine the optimal excitation and emission settings for creative new fluorescence. The estimation of monodehydroascorbate reductase (MDHAR) (EC 1. Immunofluorescence and Aggregation Analysis. SpectraMax Injector Cartridge with SmartInject™ Technology. Microplate Spectrophotometer Operator’s Manual. Nitrocefin hydrolysis was monitored at 482 nm on a SpectraMax 340 UV-visible plate reader for high-throughput assays and an HP8453 UV-visible spectrophotometer for the low-throughput assays. 5 mL of 1 M acetate buffer (pH 4. Wavelength Selection. The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurement in the visible range. Binding of capture antibodies to polystyrene beads and biotinylation. Amino acid analysis was performed on a Pico-Tag amino acid analyzer (Wat er System) as. 1 mM MnCl2 and 25 mM CHES buffer, pH 9. Absorbance at 405 nm (Abs 405) was obtained for both plates before (background) and after 1-h incubation at 37°C using a microplate reader (SpectraMax 340 PC384, Molecular Devices). , Sunnyvale, CA, USA) at 595 nm. The OD for each serum is adjusted by subtracting either the OD for spontaneous mediator release or antigen-specific mediator release. Time-resolved fluorescence was measured on the SpectraMax i3x and SpectraMax iD5 readers using preconfigured protocols in SoftMax® Pro Software (see Tables 2 and 3 for instrument-specific settings). SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. Adhesion molecule expression To determine whether baicalein could modify the oxLDL-induced adhesion molecule expression, HUVECs were grown to confluence, pretreated with baicalein for 2 hrs and stimulated with oxLDL (150 μg/ml) for. 340 to 740 nm (linear variable dichroic) 220 to 1000 nm for abs (spectrometer) Plate Formats: 6 to 384-well plates, LVis Plate with sixteen 2 µL microspots. LBA Formats for Pharmacokinetic AssaysAfter treatment, 10 μl of CCK-8 was added to each well, and the incubation was continued at 37°C for 2 h in the dark. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Please see photos. TDP-43 stress granular cells were treated with 70% ice-cold. The activity of generated thrombin was measured by the amidolysis of its substrate S-2238 and theThe purple formazan crystals formed were dissolved by adding 100 μL of DMSO to each well and absorbance was read at 570 nm in a spectrophotometer [SpectraMax 340]. measurements were performed on a SPECTRAmax-340 plate reader (Molecular Devices Inc. To correct for inter. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. Despite the relevance of adaptive immunity against equine pathogens antigen-specific T cell responses of horses are not well characterized and the lack of insight into T cell responses hampers the understanding of the pathogeneses of important diseases. 2. The Catalina. Fully tested with WarrantyThe SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). , San Jose, CA) using Softmax Pro 7 software. The levels of rheumatoid factor (RF) antibodies were measured at the Clinical Immunology Laboratory of the Sahlgrenska University Hospital or Leiden Clinical Immunology Laboratory. 05 ng/mL; progesterone: 2. 0200-7027. 2. SpectraMax® Microplate Readers and SoftMax® Pro Software. VersaMax reads 96-well and strip-well plates. SpectraMax 384 Plus. 8 (w) x 15 (d) Performance Specifications Wavelength Range: 340 – 850 nm Wavelength Selection: Monochromator, tunable 1. The plates were incubated for 4 additional hours at 37°C. Summary of Contents for Molecular Devices SPECTRAmax 340PC. Absorbance measurements were recorded using a SpectraMax 340 (Molecular Devices) at 490 nm on the indicated days (Cell Proliferation Kit I; Roche). The optical density of each well was determined immediately using a microplate reader (SpectraMax-340, Molecular Devices) set to 450 nm. Gerritsma,B. The optical density (OD) at the wavelength of 450 nm was measured with a spectrometer (SpectraMax 340, Sunnyvale, CA, USA). Afterwards, absorbance at 595 nm was measured with spectrophotometer (SpectraMax 340, Molecular Devices, USA). MOLECULAR DEVICE M5. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Apoptosis was also measured by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) assay using the TACS Blue label kit (4811-30-K, R&D Systems). Filterless Absorbance Microplate Readers provides fast & consistent results with steady temperature enabling a wide range of assays - ELISA reader, microbial growth, & protein quantitation. 2. The Molecular Devices SpectraMax 340PC384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. 5. SpectraMax Multi-Mode Microplate Readers. Manager: Yinghua Chen, PhD Contact Email: yinghua. 315 can't find zero order. Features demonstrated by the model include temperature control, a robot-compatible interface and data analysis software. ac. 0, MSD) or Spectramax 340 (Molecular Devices). Stat Fax 3200 Microplate Reader. Intra- and inter-assay coefficients of variation were lower than 8. Lightgreenglass,2 mmthick(340 nm) 9:Yellowglass,3 mmthick(405 nm) 10:Darkredglass,3 mmthick(650 nm)Bob - 06 Apr. Manager: Yinghua Chen, PhD Contact Email: yinghua. Streamline GLP/GMP compliance. Optical density was determined at 490 nm (background values measured at 690 nm were subtracted) using a SpectraMax 340 microplate reader (Molecular Devices, Sunnyvale, CA, USA). The. The cell death was calculated as follows:Absorbance was monitored at 450 nm (SpectraMax 340). Each experiment was performed in duplicate. Isolation of RNA and RT-PCR. Versteegh, Yoran H. With SOFTmax PRO, the contents of the wells in a microplate can be mixed used in the SPECTRAmax PLUS. Apoptosis was also measured by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) assay using the TACS Blue label kit (4811-30-K, R&D Systems). #ABS PLUS) Methods. Molecular Devices SpectraMax M5e Microplate Reader. 1 mL of culture media with 10% FBS. g. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. 6 (h) x 22. Italic and boldface type are used for emphasis. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. SpectraMax i3x 和 SpectraMax iD5 微孔板读板机上使用 SoftMax Pro 软件中的预设方法进行测量(有关仪器特定设 置，请参见表 2 和表 3 )。在 SpectraMax i3x 和 SpectraMax iD5 读板机上进行了微孔板优化和读取高度调整，以确保最佳 的检测灵敏度和动态范围。 数据分析SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. Spectral Range. Molecular Device M5e. StakMax. 5 hours, and is measured in a 96-well plate spectrophotometer at 405 nm (SpectraMax 340; Molecular Devices LLC, Sunnyvale, CA, USA). Page 1 ® SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0049 Rev. Awareness Technology, Inc. The metals were preincubated with A for 5 min when added before heme. 2. 1. chen@case. Absorbance was measured at 490/650 nm by using a SpectraMax 340 microplate spectrophotometer® (Molecular Devices, Sunnyvale, CA, U. After 24 h, 48 h, 72 h or 96 h, cell viability was measured by CellTiter 96® AQueous One Solution cell proliferation assay (Promega, Madison, WI, USA). The adapter is. Thin layer chromatography (TLC) was. TheSpectraMax®Plus384addstheabilitytoreadcuvettes. Values were determined according to a standard curve (log/log curve-fit). PGD2 concentrations in samples from treated cell cultures were determined using the linear range of a standard curve and are presented as percentages of the PGD2 levels measured in the solvent controls. Key benefits • User-upgradeable application modules including cellular imaging SpectraTest ABS1 Absorbance Validation Plate. Cell culture 3T3 fibroblasts and RAW 264. Read up to 6 wavelengths in endpoint or kinetic mode for 96- and 384-well plates, and perform spectral scans to determine. This is equivalent to having 511 built-in filters,Results: A total of 64% of the subjects reported improved quality of sleep during treatment with naloxone at a dose of 40 ng per 24 hours as compared with 9% with sham treatment (P=0. Sandwich ELISAs were used to measure insulin (DY8056, R&D Systems, Minneapolis, MN, USA). The absorbance was measured at 340 nm for 2 min at a 15 s time interval. 20. Microplate Reader Shelf. About GMI provides new and GMI Certified Pre-Owned scientific instrumentation and service to research universities, environmental labs, forensic investigators, biotechnology and pharmaceutical companies, hospitals, clinics and analytical laboratories, in the United. Introduction The SpectraMax® 340PC384 Reader from Molecular Devices provides everything needed to measure absorbance in the visible range, including temperature. . Statistical analysis The significant differences between the different strains in the adherence assays were analyzed using the Student’s t -test. Although not statistically significant, pain was reduced by 2 NRS steps or more during supplemental treatment with naloxone in 36% of subjects when. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Dissolve 13. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. 6 g sodium acetate trihydrate and 2. Enzyme activities at high concentration were analyzed using a SpectraMAX 340 plate reader (Molecular Devices) at 380 nm in the kinetic mode at 37°C. Donor filter: 620 nm. Near bottom. ZERO BIAS - scores, article reviews, protocol conditions and moreAbsorbance was measured at 450 nm with ELISA Reader (SpectraMax 340, Molecular Device Co. Media in plates were withdrawn by pipetting and 200 μl of DMSO was added to each well to solubilize the formazan crystals. 00. 00.